Exam code:8585
Microorganisms, enzymes & food
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Food spoilage can be defined as:
Loss of food quality that causes food to become inedible
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Food spoilage can occur due to:
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activity of microorganisms
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enzyme activity within the food
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Microorganisms and food spoilage
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Microorganisms are very small organisms that can only be seen using a microscope, e.g.
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bacteria
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fungi, such as yeast and mould
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Not all microorganisms are harmful, but some can cause food spoilage and may result in food poisoning if consumed

Conditions for microorganism growth
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In order to grow, microorganisms need specific conditions, including:
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correct temperature
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Conditions need to be warm enough for microorganisms to survive without being hot enough to destroy them
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water
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Moisture needs to be present in the food or in the surrounding air
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food
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Microorganisms need nutrients to enable them to survive and reproduce
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correct pH
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Most microorganisms cannot survive in highly acidic or highly alkaline conditions
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In order to reach harmful levels, microorganisms need a sufficient length of time under suitable conditions
Preventing microorganism growth
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Microorganism growth can be reduced by making the conditions inhospitable; this can be achieved by:
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reducing the temperature, e.g. by putting food in the fridge or freezer
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increasing the temperature by cooking food
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reducing the length of time for which food is left at room temperature
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providing an acidic environment, e.g. by pickling
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removing water, e.g. by drying, salting or adding lots of sugar
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High risk foods for microorganism growth
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Foods that spoil easily due to microorganism growth are known as high risk foods
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These foods provide an environment that aids the growth of microorganisms; they are often
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moist
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high in protein, which provides food for microorganisms
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High risk foods are generally ‘ready-to-eat’, meaning that they will not undergo a further heating process before consumption that might destroy microorganisms
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Examples of high risk foods include:
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cooked meat and fish
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dairy products, such as cheese and milk
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some forms of egg, and foods that contain egg, e.g. mayonnaise
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precooked shellfish, e.g. mussels and prawns
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meat products, including gravy, stock and sauces
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cooked rice
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Enzymes and food spoilage
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Enzymes are biological catalysts made of protein
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The activity of enzymes that are already present inside foods can lead to enzymic browning
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Food spoilage due to enzymes can be prevented by denaturing enzymes to reduce their activity; this can be achieved by:
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heating, e.g. by blanching vegetables in boiling water for a few minutes before freezing
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adding an acid, e.g. putting lemon juice on sliced apple
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