Biology_Alevel_Ocr
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4-1-communicable-diseases-disease-prevention-and-the-immune-system16 主题
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4-1-1-common-pathogens-and-communicable-diseases
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4-1-2-transmission-of-communicable-pathogens
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4-1-3-plant-defences-against-pathogens
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4-1-4-non-specific-immune-responses
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4-1-5-phagocytes
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4-1-6-blood-cells
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4-1-7-the-t-lymphocyte-response
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4-1-8-the-b-lymphocyte-response
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4-1-9-primary-and-secondary-immune-responses
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4-1-10-antibodies
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4-1-11-opsonins-agglutinins-and-anti-toxins
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4-1-12-types-of-immunity
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4-1-13-autoimmune-diseases
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4-1-14-principles-of-vaccination
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4-1-15-sources-of-medicine
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4-1-16-antibiotics
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4-1-1-common-pathogens-and-communicable-diseases
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4-2-biodiversity10 主题
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4-2-1-biodiversity
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4-2-2-sampling-to-determine-biodiversity
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4-2-3-practical-investigating-biodiversity-using-sampling
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4-2-4-measuring-species-richness-and-species-evenness
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4-2-5-simpsons-index
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4-2-6-genetic-diversity
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4-2-7-factors-affecting-biodiversity
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4-2-8-reasons-for-maintaining-biodiversity
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4-2-9-methods-of-maintaining-biodiversity
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4-2-10-conservation-agreements
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4-2-1-biodiversity
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4-3-classification-and-evolution15 主题
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4-3-1-classification-of-species
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4-3-2-binomial-system
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4-3-3-classification-of-the-three-domains
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4-3-4-classification-of-the-five-kingdoms
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4-3-5-classification-and-phylogeny
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4-3-6-evidence-of-evolution
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4-3-7-types-of-variation
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4-3-8-standard-deviation
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4-3-9-variation-t-test-method
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4-3-10-variation-t-test-worked-example
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4-3-11-spearmans-rank-correlation
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4-3-12-adaptation
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4-3-13-natural-selection
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4-3-14-evolution-of-resistance
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4-3-15-consequences-of-resistance
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4-3-1-classification-of-species
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5-1-communication-and-homeostasis4 主题
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5-2-excretion10 主题
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5-2-1-the-importance-of-excretion
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5-2-2-the-mammalian-liver-structure
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5-2-3-the-mammalian-liver-function
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5-2-4-the-liver-under-the-microscope
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5-2-5-the-mammalian-kidney-structure
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5-2-6-the-mammalian-kidney-function
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5-2-7-the-kidney-under-the-microscope
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5-2-8-osmoregulation
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5-2-9-kidney-failure
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5-2-10-excretory-products-and-medical-diagnosis
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5-2-1-the-importance-of-excretion
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5-3-neuronal-communication9 主题
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5-4-hormonal-communication4 主题
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5-5-plant-and-animal-responses16 主题
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5-5-1-plant-responses
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5-5-2-investigating-phototropism-and-geotropism
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5-5-3-plant-hormones
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5-5-4-auxins-and-apical-dominance
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5-5-5-gibberellin
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5-5-6-practical-effect-of-plant-hormones-on-growth
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5-5-7-commercial-use-of-plant-hormones
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5-5-8-mammalian-nervous-system
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5-5-9-the-human-brain
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5-5-10-reflex-actions
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5-5-11-coordination-of-responses
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5-5-12-factors-affecting-heart-rate
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5-5-13-investigating-factors-affecting-heart-rate
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5-5-14-mammalian-muscle-structure
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5-5-15-transmission-across-a-neuromuscular-junction
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5-5-16-the-sliding-filament-model
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5-5-1-plant-responses
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5-6-photosynthesis10 主题
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5-6-1-photosynthesis-and-respiration
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5-6-2-chloroplast-structure-and-function
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5-6-3-photosynthetic-pigments
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5-6-4-practical-investigating-photosynthetic-pigments-with-chromatography
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5-6-5-the-light-dependent-stage
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5-6-6-using-the-products-of-the-light-dependent-reaction
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5-6-7-the-light-independent-stage
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5-6-8-uses-of-triose-phosphate
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5-6-9-factors-affecting-the-rate-of-photosynthesis
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5-6-10-practical-investigating-factors-affecting-the-rate-of-photosynthesis
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5-6-1-photosynthesis-and-respiration
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5-7-respiration14 主题
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5-7-14-practical-respirometer
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5-7-1-the-need-for-cellular-respiration
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5-7-2-structure-of-the-mitochondrion
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5-7-3-the-four-stages-in-aerobic-respiration
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5-7-4-glycolysis
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5-7-5-the-link-reaction
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5-7-6-the-krebs-cycle
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5-7-7-the-role-of-coenzymes
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5-7-8-oxidative-phosphorylation
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5-7-9-anaerobic-respiration
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5-7-10-energy-yield-of-aerobic-vs-anaerobic-respiration
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5-7-11-practical-investigating-the-rate-of-respiration
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5-7-12-respiratory-substrates
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5-7-13-respiratory-quotient-rq
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5-7-14-practical-respirometer
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6-1-cellular-control7 主题
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6-2-patterns-of-inheritance13 主题
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6-2-1-key-terms-in-genetics
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6-2-2-variation-phenotype
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6-2-3-variation-sexual-reproduction
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6-2-4-predicting-inheritance-monohybrid-crosses
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6-2-5-predicting-inheritance-dihybrid-crosses
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6-2-6-predicting-inheritance-identifying-linkage
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6-2-7-predicting-inheritance-identifying-epistasis
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6-2-8-predicting-inheritance-chi-squared-test
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6-2-9-continuous-and-discontinuous-variation
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6-2-10-factors-affecting-evolution
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6-2-11-the-hardy-weinberg-principle
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6-2-12-isolation-and-speciation
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6-2-13-artificial-selection
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6-2-1-key-terms-in-genetics
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6-3-manipulating-genomes11 主题
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6-3-1-dna-sequencing
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6-3-2-comparing-genomes
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6-3-3-non-coding-dna-and-regulatory-genes
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6-3-4-synthetic-biology
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6-3-5-polymerase-chain-reaction
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6-3-6-electrophoresis
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6-3-7-dna-profiling
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6-3-8-genetic-engineering
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6-3-9-genetic-engineering-techniques
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6-3-10-uses-of-genetic-engineering
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6-3-11-gene-therapy
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6-3-1-dna-sequencing
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6-4-cloning-and-biotechnology14 主题
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6-4-1-natural-clones-in-plants
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6-4-2-producing-cuttings
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6-4-3-production-of-artificial-clones-in-plants
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6-4-4-uses-of-plant-cloning
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6-4-5-natural-clones-in-animals
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6-4-6-production-of-artificial-clones-in-animals
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6-4-7-uses-of-animal-cloning
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6-4-8-microorganisms-and-biotechnology
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6-4-9-microorganisms-and-food-production
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6-4-10-culturing-microorganisms
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6-4-11-batch-and-continuous-fermentation
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6-4-12-standard-growth-curve-of-microorganisms
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6-4-13-factors-affecting-the-growth-of-microorganisms
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6-4-14-immobilised-enzymes-in-biotechnology
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6-4-1-natural-clones-in-plants
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6-5-ecosystems7 主题
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6-6-populations-and-sustainability6 主题
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1-1-practical-skills-written-assessment7 主题
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1-2-practical-skills-endorsement-assessment16 主题
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1-2-1-practical-ethical-use-of-organisms
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1-2-2-practical-aseptic-techniques
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1-2-3-practical-dissection-of-gas-exchange-surfaces-in-fish-and-insects
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1-2-4-drawing-cells-from-blood-smears
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1-2-5-practical-investigating-biodiversity-using-sampling
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1-2-6-practical-data-loggers-and-computer-modelling
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1-2-7-practical-investigating-the-rate-of-diffusion
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1-2-8-practical-investigating-water-potential
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1-2-9-practical-factors-affecting-membrane-structure-and-permeability
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1-2-10-biochemical-tests-reducing-sugars-and-starch
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1-2-11-biochemical-tests-lipids
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1-2-12-biochemical-tests-proteins
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1-2-13-chromatography
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1-2-14-serial-dilutions
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1-2-15-practical-investigating-the-rate-of-transpiration
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1-2-16-practical-using-a-light-microscope
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1-2-1-practical-ethical-use-of-organisms
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2-1-cell-structure9 主题
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2-2-biological-molecules17 主题
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2-2-1-properties-of-water
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2-2-2-monomers-and-polymers
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2-2-3-monosaccharides
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2-2-4-the-glycosidic-bond
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2-2-5-polysaccharides
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2-2-6-biochemical-tests-reducing-sugars-and-starch
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2-2-7-lipids-and-ester-bonds
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2-2-8-lipids-structure-and-function
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2-2-9-biochemical-tests-lipids
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2-2-10-amino-acids-and-peptide-bonds
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2-2-11-protein-structure
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2-2-12-globular-proteins
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2-2-13-fibrous-proteins
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2-2-14-inorganic-ions
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2-2-15-biochemical-tests-proteins
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2-2-16-finding-the-concentration-of-a-substance
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2-2-17-chromatography
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2-2-1-properties-of-water
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2-3-nucleotides-and-nucleic-acids8 主题
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2-4-enzymes9 主题
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2-4-1-the-role-of-enzymes
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2-4-2-enzyme-action
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2-4-3-enzyme-activity-ph
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2-4-4-enzyme-activity-temperature
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2-4-5-enzyme-activity-enzyme-concentration
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2-4-6-enzyme-activity-substrate-concentration
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2-4-7-enzyme-activity-enzyme-inhibitors
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2-4-8-coenzymes-cofactors-and-prosthetic-groups
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2-4-9-practical-measuring-enzyme-activity
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2-4-1-the-role-of-enzymes
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2-5-biological-membranes9 主题
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2-5-1-the-cell-surface-membrane
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2-5-2-membrane-structure-and-permeability
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2-5-3-diffusion-and-facilitated-diffusion
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2-5-4-practical-investigating-the-rate-of-diffusion
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2-5-5-active-transport
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2-5-6-endocytosis-and-exocytosis
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2-5-7-osmosis
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2-5-8-osmosis-in-animal-and-plant-cells
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2-5-9-practical-investigating-water-potential
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2-5-1-the-cell-surface-membrane
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2-6-cell-division-cell-diversity-and-cellular-organisation11 主题
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2-6-1-the-cell-cycle
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2-6-2-the-stages-of-mitosis
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2-6-3-identifying-mitosis-in-plant-cells
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2-6-4-the-significance-of-mitosis
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2-6-5-the-stages-of-meiosis
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2-6-6-the-significance-of-meiosis
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2-6-7-specialised-cells
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2-6-8-the-organisation-of-cells
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2-6-9-stem-cells
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2-6-10-stem-cells-in-animals-and-plants
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2-6-11-the-use-of-stem-cells
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2-6-1-the-cell-cycle
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3-1-exchange-surfaces7 主题
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3-2-transport-in-animals12 主题
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3-2-1-the-need-for-transport-systems-in-animals
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3-2-2-circulatory-systems
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3-2-3-blood-vessels
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3-2-4-tissue-fluid
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3-2-5-the-mammalian-heart
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3-2-6-practical-mammalian-heart-dissection
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3-2-7-the-cardiac-cycle
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3-2-8-cardiac-output
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3-2-9-heart-action-initiation-and-control
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3-2-10-electrocardiograms-ecgs
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3-2-11-the-role-of-haemoglobin
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3-2-12-adult-and-fetal-haemoglobin
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3-2-1-the-need-for-transport-systems-in-animals
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3-3-transport-in-plants11 主题
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3-3-1-the-need-for-transport-systems-in-plants
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3-3-2-the-xylem-and-phloem
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3-3-3-the-xylem
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3-3-4-the-phloem
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3-3-5-transverse-sections-stems-roots-and-leaves
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3-3-6-the-process-of-transpiration
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3-3-7-transpiration-in-plants
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3-3-8-practical-investigating-the-rate-of-transpiration
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3-3-9-translocation
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3-3-10-the-mass-flow-hypothesis
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3-3-11-the-adaptations-of-xerophytic-and-hydrophytic-plants
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3-3-1-the-need-for-transport-systems-in-plants
4-3-11-spearmans-rank-correlation
Spearman’s Rank Correlation
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Spearman’s rank correlation determines whether there is correlation between variables that don’t show a normal distribution
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Method:
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Step 1: Create a scatter graph and identify possible linear correlation
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Step 2: State a null hypothesis
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Step 3: Use the following equation to work out Spearman’s rank correlation coefficient r

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Where:
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rs = spearman’s rank coefficient
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D = difference in rank
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n = number of samples
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Step 4: Refer to a table that relates critical values of rs to levels of probability
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If the value calculated for Spearman’s rank is greater than the critical value for the number of samples in the data ( n ) at the 0.05 probability level (p), then the null hypothesis can be rejected, meaning there is a correlation between two variables
Worked Example
A student conducted an experiment using quadrats to measure the abundance of different plant species in a neglected allotment. They wanted to see if there was correlation between the abundance of species C and D. When they looked at their data and plotted a scatter graph they saw some correlation.Investigate the possible correlation using Spearman’s rank correlation coefficient.

Scatter graph showing the correlation between the abundance of species C and species D
Answer:
As the data was not normally distributed they decided to use Spearman’s rank correlation coefficient.
Null hypothesis: there is no correlation between the abundance of species A and species B.
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n = 10 as there are 10 quadrat samples

Step 1: Rank each set of data (rank 1 being the smallest data figure)
Step 2: Find the difference in rank between the two species, D
Step 3: Square the difference in rank, D2 (= 6)
Step 4: Substitute the appropriate numbers into the equation (remember n = 10)

Step 5: Refer to a table that relates values of rs to probability. Look for the 0.05 probability level with n = 10

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As Rs = 0.964, it is greater than the critical value of 0.65. The null hypothesis can be rejected, there is a genuine positive correlation between the abundance of species A and B
Examiner Tips and Tricks
You will be provided with the formula for Spearman’s rank correlation in the exam. You need to be able to carry out the calculation to test for correlation, as you could be asked to do this in the exam. You should understand when it is appropriate to use the different statistical tests that crop up in this topic, and the conditions in which each is valid.Correlation does not always mean causation. Just because there is a correlation between the abundance of species A and species B it does not mean that the presence of species A causes the presence of species B.