Biology_A-level_Cie
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1-1-the-microscope-in-cell-studies5 主题
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1-2-cells-as-the-basic-units-of-living-organisms5 主题
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2-1-testing-for-biological-molecules3 主题
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2-2-carbohydrates-and-lipids8 主题
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2-3-proteins6 主题
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2-4-water2 主题
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3-1-mode-of-action-of-enzymes5 主题
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3-2-factors-that-affect-enzyme-action8 主题
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4-1-fluid-mosaic-membranes4 主题
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4-2-movement-into-and-out-of-cells12 主题
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diffusion
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osmosis
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active-transport
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endocytosis-and-exocytosis
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investigating-transport-processes-in-plants
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investigating-diffusion
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surface-area-to-volume-ratios
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investigating-surface-area
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estimating-water-potential-in-plants
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osmosis-in-plant-cells
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osmosis-in-animals
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comparing-osmosis-in-plants-and-animals
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diffusion
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5-1-replication-and-division-of-nuclei-and-cells6 主题
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5-2-chromosome-behaviour-in-mitosis2 主题
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6-1-structure-of-nucleic-acids-and-replication-of-dna4 主题
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6-2-protein-synthesis5 主题
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7-1-structure-of-transport-tissues4 主题
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7-2-transport-mechanisms7 主题
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8-1-the-circulatory-system7 主题
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8-2-transport-of-oxygen-and-carbon-dioxide5 主题
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8-3-the-heart4 主题
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9-1-the-gas-exchange-system6 主题
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10-1-infectious-diseases3 主题
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10-2-antibiotics3 主题
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11-1-the-immune-system4 主题
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11-2-antibodies-and-vaccination6 主题
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12-1-energy5 主题
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12-2-respiration11 主题
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aerobic-respiration-the-krebs-cycle
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aerobic-respiration-role-of-nad-and-fad
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aerobic-respiration-oxidative-phosphorylation
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anaerobic-respiration
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energy-yield-aerobic-and-anaerobic-respiration
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anaerobic-adaptation-of-rice
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aerobic-respiration-effect-of-temperature-and-substrate-concentration
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structure-and-function-of-mitochondria
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the-four-stages-in-aerobic-respiration
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aerobic-respiration-glycolysis
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aerobic-respiration-the-link-reaction
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aerobic-respiration-the-krebs-cycle
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13-1-photosynthesis-as-an-energy-transfer-process8 主题
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13-2-investigation-of-limiting-factors2 主题
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14-1-homeostasis-in-mammals8 主题
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14-2-homeostasis-in-plants3 主题
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15-1-control-and-coordination-in-mammals12 主题
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the-endocrine-system
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the-nervous-system
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neurones
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sensory-receptor-cells
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sequence-of-events-resulting-in-an-action-potential
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transmission-of-nerve-impulses
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speed-of-conduction-of-impulses
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the-refractory-period
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cholinergic-synapses
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stimulating-contraction-in-striated-muscle
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ultrastructure-of-striated-muscle
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sliding-filament-model-of-muscular-contraction
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the-endocrine-system
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15-2-control-and-coordination-in-plants3 主题
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16-1-passage-of-information-from-parents-to-offspring5 主题
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16-2-the-roles-of-genes-in-determining-the-phenotype7 主题
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16-3-gene-control3 主题
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17-1-variation4 主题
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17-2-natural-and-artificial-selection7 主题
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17-3-evolution2 主题
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18-1-classification5 主题
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18-2-biodiversity7 主题
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18-3-conservation6 主题
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19-1-principles-of-genetic-technology11 主题
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19-2-genetic-technology-applied-to-medicine4 主题
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19-3-genetically-modified-organisms-in-agriculture2 主题
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1-1-the-microscope-in-cell-studies
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1-2-cells-as-the-basic-units-of-living-organisms
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2-1-testing-for-biological-molecules
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2-2-carbohydrates-and-lipids
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2-3-proteins
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2-4-water
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3-1-mode-of-action-of-enzymes
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3-2-factors-that-affect-enzyme-action
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4-1-fluid-mosaic-membranes
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4-2-movement-into-and-out-of-cells
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5-1-replication-and-division-of-nuclei-and-cells
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5-2-chromosome-behaviour-in-mitosis
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6-1-structure-of-nucleic-acids-and-replication-of-dna
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6-2-protein-synthesis
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7-1-structure-of-transport-tissues
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7-2-transport-mechanisms
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8-1-the-circulatory-system
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8-2-transport-of-oxygen-and-carbon-dioxide
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8-3-the-heart
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9-1-the-gas-exchange-system
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10-1-infectious-diseases
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10-2-antibiotics
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11-1-the-immune-system
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11-2-antibodies-and-vaccination
testing-for-distribution-and-abundance
Testing for distribution & abundance
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The distribution of a species describes how it is spread throughout the ecosystem
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The abundance of a species is the number of individuals of that species
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The distribution and abundance of a species in an area can be assessed using different practical methods:
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Frame quadrats
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Line and belt transects
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Mark-release-recapture
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Frame quadrats
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Some ecosystems are very complex with large numbers of different species of different sizes
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For the sake of logistics, sampling is often used to estimate the distribution and abundance of species
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When carrying out sampling, square frames called quadrats can be used to mark off the area being sampled
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Quadrats of different sizes can be used depending on what is being measured and what is most suitable in the space the samples are being made in
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These are usually made of wood or metal and measure 25 cm × 25 cm, 50 cm × 50 cm or 1 m × 1 m
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Quadrats must be laid randomly in the area to avoid sampling bias
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This random sampling can be done by converting the sampling area into a grid format and labelling each square on the grid with a number
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Then a random number generator is used to pick the sample points
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Once the quadrat has been laid on the chosen sample point the abundance of all the different species present can be recorded
Results from quadrats
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The results from the quadrats can be used to calculate the predicted frequency and density of a species within an area
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Species frequency is the probability that the species will be found within any quadrat in the sample area
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The number of quadrats that the species was present in is divided by the total number of quadrats and then multiplied by 100
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For example, if bluebells were found in 18 out of 50 quadrats the species frequency would be (18÷50) x 100 = 36%
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Species density indicates how many individuals of that species there are per unit area
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The number of individuals counted across all quadrats is divided by the total area of all the quadrats
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For example, if 107 bluebells were found across 50 quadrats that are 1m2 each the species density would be 107/50 = 2.14 individuals per m2
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It can sometimes be difficult to count individual plants or organisms. When this is the case percentage cover of the species within the quadrat can be estimated instead
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The quadrat is divided into 100 smaller squares
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The number of squares the species is found in is equivalent to its percentage cover in that quadrat
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For example, if grass is found in 89 out of 100 squares in the quadrat then it has a percentage cover of 89%
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Line and belt transects
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Throughout some areas, there can be changes in the physical conditions
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For example, there may be changes in altitude, soil pH or light intensity
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When investigating the species distribution in these kinds of areas, systematic sampling is more appropriate
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Methods using transects can help show how species distribution changes with the different physical conditions in the area
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A transect is a line represented by a measuring tape, along which sample are taken
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For a line transect:
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Lay out a measuring tape in a straight line across the sample area
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At equal distances along the tape record the identity of the organisms that touch the line. For example, every 2m
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This produces qualitative data
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For a belt transect:
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Place quadrats at regular intervals along the tape and record the abundance of each species within each quadrat
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This produces quantitative data
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Mark-release-recapture
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The methods above are only useful for stationary organisms
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Different methods are required for estimating the number of individuals in a population of mobile animals
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The mark-release-recapture method is used in conjunction with the Lincoln Index
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For a single species in the area:
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The first large sample is taken. As many individuals as possible are captured / collected, counted and marked in a way that won’t affect their survival
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They are returned to their habitat and allowed to mix randomly with the rest of the population
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When a sufficient amount of time has passed another large sample is captured
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The numbers of marked and unmarked individuals within the sample are counted
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The proportion of marked to unmarked individuals is used to calculate an estimate of the population size
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The formula for the calculation is:
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Where:
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N = population estimate
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n1 = number of marked individuals released
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n2 = number of individuals in the second sample (marked and unmarked)
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m2 = number of marked individuals in the second sample
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Worked Example
Lincoln Index with mark-release-recapture
Scientists wanted to investigate the abundance of leafhoppers (Cicadellidae species) in a small grassy meadow. They used sweep nets to catch a large sample of leafhoppers from the meadow.
