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Biology_A-level_Cie

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  1. 1-1-the-microscope-in-cell-studies
    5 主题
  2. 1-2-cells-as-the-basic-units-of-living-organisms
    5 主题
  3. 2-1-testing-for-biological-molecules
    3 主题
  4. 2-2-carbohydrates-and-lipids
    8 主题
  5. 2-3-proteins
    6 主题
  6. 2-4-water
    2 主题
  7. 3-1-mode-of-action-of-enzymes
    5 主题
  8. 3-2-factors-that-affect-enzyme-action
    8 主题
  9. 4-1-fluid-mosaic-membranes
    4 主题
  10. 4-2-movement-into-and-out-of-cells
    12 主题
  11. 5-1-replication-and-division-of-nuclei-and-cells
    6 主题
  12. 5-2-chromosome-behaviour-in-mitosis
    2 主题
  13. 6-1-structure-of-nucleic-acids-and-replication-of-dna
    4 主题
  14. 6-2-protein-synthesis
    5 主题
  15. 7-1-structure-of-transport-tissues
    4 主题
  16. 7-2-transport-mechanisms
    7 主题
  17. 8-1-the-circulatory-system
    7 主题
  18. 8-2-transport-of-oxygen-and-carbon-dioxide
    5 主题
  19. 8-3-the-heart
    4 主题
  20. 9-1-the-gas-exchange-system
    6 主题
  21. 10-1-infectious-diseases
    3 主题
  22. 10-2-antibiotics
    3 主题
  23. 11-1-the-immune-system
    4 主题
  24. 11-2-antibodies-and-vaccination
    6 主题
  25. 12-1-energy
    5 主题
  26. 12-2-respiration
    11 主题
  27. 13-1-photosynthesis-as-an-energy-transfer-process
    8 主题
  28. 13-2-investigation-of-limiting-factors
    2 主题
  29. 14-1-homeostasis-in-mammals
    8 主题
  30. 14-2-homeostasis-in-plants
    3 主题
  31. 15-1-control-and-coordination-in-mammals
    12 主题
  32. 15-2-control-and-coordination-in-plants
    3 主题
  33. 16-1-passage-of-information-from-parents-to-offspring
    5 主题
  34. 16-2-the-roles-of-genes-in-determining-the-phenotype
    7 主题
  35. 16-3-gene-control
    3 主题
  36. 17-1-variation
    4 主题
  37. 17-2-natural-and-artificial-selection
    7 主题
  38. 17-3-evolution
    2 主题
  39. 18-1-classification
    5 主题
  40. 18-2-biodiversity
    7 主题
  41. 18-3-conservation
    6 主题
  42. 19-1-principles-of-genetic-technology
    11 主题
  43. 19-2-genetic-technology-applied-to-medicine
    4 主题
  44. 19-3-genetically-modified-organisms-in-agriculture
    2 主题
  45. 1-1-the-microscope-in-cell-studies
  46. 1-2-cells-as-the-basic-units-of-living-organisms
  47. 2-1-testing-for-biological-molecules
  48. 2-2-carbohydrates-and-lipids
  49. 2-3-proteins
  50. 2-4-water
  51. 3-1-mode-of-action-of-enzymes
  52. 3-2-factors-that-affect-enzyme-action
  53. 4-1-fluid-mosaic-membranes
  54. 4-2-movement-into-and-out-of-cells
  55. 5-1-replication-and-division-of-nuclei-and-cells
  56. 5-2-chromosome-behaviour-in-mitosis
  57. 6-1-structure-of-nucleic-acids-and-replication-of-dna
  58. 6-2-protein-synthesis
  59. 7-1-structure-of-transport-tissues
  60. 7-2-transport-mechanisms
  61. 8-1-the-circulatory-system
  62. 8-2-transport-of-oxygen-and-carbon-dioxide
  63. 8-3-the-heart
  64. 9-1-the-gas-exchange-system
  65. 10-1-infectious-diseases
  66. 10-2-antibiotics
  67. 11-1-the-immune-system
  68. 11-2-antibodies-and-vaccination
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testing-for-distribution-and-abundance

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Testing for distribution & abundance

  • The distribution of a species describes how it is spread throughout the ecosystem

  • The abundance of a species is the number of individuals of that species

  • The distribution and abundance of a species in an area can be assessed using different practical methods:

    • Frame quadrats

    • Line and belt transects

    • Mark-release-recapture

Frame quadrats

  • Some ecosystems are very complex with large numbers of different species of different sizes

  • For the sake of logistics, sampling is often used to estimate the distribution and abundance of species

  • When carrying out sampling, square frames called quadrats can be used to mark off the area being sampled

  • Quadrats of different sizes can be used depending on what is being measured and what is most suitable in the space the samples are being made in

    • These are usually made of wood or metal and measure 25 cm × 25 cm, 50 cm × 50 cm or 1 m × 1 m

  • Quadrats must be laid randomly in the area to avoid sampling bias

    • This random sampling can be done by converting the sampling area into a grid format and labelling each square on the grid with a number

    • Then a random number generator is used to pick the sample points

  • Once the quadrat has been laid on the chosen sample point the abundance of all the different species present can be recorded

Results from quadrats

  • The results from the quadrats can be used to calculate the predicted frequency and density of a species within an area

  • Species frequency is the probability that the species will be found within any quadrat in the sample area

    • The number of quadrats that the species was present in is divided by the total number of quadrats and then multiplied by 100

    • For example, if bluebells were found in 18 out of 50 quadrats the species frequency would be (18÷50) x 100 = 36%

  • Species density indicates how many individuals of that species there are per unit area

    • The number of individuals counted across all quadrats is divided by the total area of all the quadrats

    • For example, if 107 bluebells were found across 50 quadrats that are 1m2 each the species density would be 107/50 = 2.14 individuals per m2

  • It can sometimes be difficult to count individual plants or organisms. When this is the case percentage cover of the species within the quadrat can be estimated instead

    • The quadrat is divided into 100 smaller squares

    • The number of squares the species is found in is equivalent to its percentage cover in that quadrat

    • For example, if grass is found in 89 out of 100 squares in the quadrat then it has a percentage cover of 89%

Grid diagram showing species distribution in quadrats: brown for neither, green for species 1, dark green for species 2 overlaying species 1.
Percentage cover of grass in a quadrat – There may be some squares lacking any species and other squares with multiple species overlying one another. Therefore the total percentage cover of a single quadrat can sometimes be over or under 100%

Line and belt transects

  • Throughout some areas, there can be changes in the physical conditions

    • For example, there may be changes in altitude, soil pH or light intensity

  • When investigating the species distribution in these kinds of areas, systematic sampling is more appropriate

  • Methods using transects can help show how species distribution changes with the different physical conditions in the area

    • A transect is a line represented by a measuring tape, along which sample are taken

  • For a line transect:

    • Lay out a measuring tape in a straight line across the sample area

    • At equal distances along the tape record the identity of the organisms that touch the line. For example, every 2m

    • This produces qualitative data

  • For a belt transect:

    • Place quadrats at regular intervals along the tape and record the abundance of each species within each quadrat

    • This produces quantitative data

Diagram showing line transect and belt transect methods for recording organisms, with brief explanations of sampling and abundance recording.
A line transect and belt transect is carried out in the field

Mark-release-recapture

  • The methods above are only useful for stationary organisms

  • Different methods are required for estimating the number of individuals in a population of mobile animals

  • The mark-release-recapture method is used in conjunction with the Lincoln Index

  • For a single species in the area:

    • The first large sample is taken. As many individuals as possible are captured / collected, counted and marked in a way that won’t affect their survival

    • They are returned to their habitat and allowed to mix randomly with the rest of the population

    • When a sufficient amount of time has passed another large sample is captured

    • The numbers of marked and unmarked individuals within the sample are counted

    • The proportion of marked to unmarked individuals is used to calculate an estimate of the population size

    • The formula for the calculation is:

 straight N equals fraction numerator straight n subscript 1 cross times straight n subscript 2 over denominator straight m subscript 2 end fraction

  • Where:

    • N = population estimate

    • n1 = number of marked individuals released

    • n2 = number of individuals in the second sample (marked and unmarked)

    • m2 = number of marked individuals in the second sample

Worked Example

Lincoln Index with mark-release-recapture

Scientists wanted to investigate the abundance of leafhoppers (Cicadellidae species) in a small grassy meadow. They used sweep nets to catch a large sample of leafhoppers from the meadow.

A person holds a large white butterfly net with a metal handle over grassy ground, near tall vegetation.
A sweep net used to collect insects (Kieren, CC BY 3.0, via Wikimedia Commons)
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