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Biology AS Edexcel Snab Revision

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Exam code:8BN0

Importance of Water & Inorganic Ions to Plants

  • Plant cells perform a variety of different functions

  • In order to perform these functions efficiently, the plant requires water and inorganic ions (minerals)

  • They are absorbed through the root hairs on the root and travel up the stem in xylem vessels

  • A plant will show certain symptoms (e.g. yellow leaves, stunted growth) when there is a deficiency in any one of these substances

Water

  • Important component required for photosynthesis

  • Provides a transport medium for minerals

  • Maintains turgidity in plant cells though pressure in cell vacuoles

  • Regulates temperature – to ensure that enzymes can function at their optimum rate

Magnesium ions

  • Important requirement for the production of chlorophyll

  • This provides the green colour of stems and leaves and is essential for photosynthesis

Nitrate ions

  • Without nitrate ions, the plant would be unable to synthesise DNA, proteins and chlorophyll

    • Enzymes are important proteins for which nitrate ions are needed

  • These molecules are essential for plant growth, as well as the production of fruit and seeds

Calcium ions

  • These form important cell wall components

  • Plants require calcium ions for proper growth

Mineral deficiencies in plants

Diagram showing the importance of magnesium and nitrate ions for plants

Practical: Investigating Plant Mineral Deficiencies

  • The following experiment could be done with any one of the mineral ions mentioned earlier

  • For this example, the focus will be on investigating the effect of a calcium deficiency on plants

Apparatus

  • Nutrient broths

  • Test tubes

  • Seedlings

  • Aluminium foil

  • Mass balance

Method

  • Prepare three nutrient broths containing every mineral that a plant requires, but with different concentrations of calcium ions in each (high, medium and low)

    • Label three test tubes for each of the nutrient broths (three ‘high’, three ‘medium’ and three ‘low’ = nine in total)

  • Take nine seedlings, ensuring that they are from the same plant and are the same age, and record the mass of each

  • Place one seedling on top of each test tube, suspending the roots in the nutrient broth

  • Cover the test tubes with aluminium foil to keep light away from the broth

  • Place the test tubes near a source of light and leave them for a few days

  • Remove each plant from the broth and carefully blot it dry before measuring the mass again

  • Record the end mass and use that to calculate the mean change in mass of the plants for each of the different nutrient broths

  • Make a note of any physical differences between the plants of the different groups

Variation of the practical

  • Another variation of the experiment mentioned above, is to investigate the effect on plant growth when the mineral is completely lacking

  • In this experiment you would have broths that contains all of the minerals, except the one that is being investigated

  • There would also need to be two control broths

    • One would contain all the minerals

    • The other would contain no minerals

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