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Biology AS CIE

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  1. 1-cell-structure
    10 主题
  2. 2-biological-molecules
    19 主题
  3. 3-enzymes
    13 主题
  4. 4-cell-membranes-and-transport
    16 主题
  5. 5-the-mitotic-cell-cycle
    8 主题
  6. 6-nucleic-acids-and-protein-synthesis
    9 主题
  7. 7-transport-in-plants
    11 主题
  8. 8-transport-in-mammals
    16 主题
  9. 9-gas-exchange
    6 主题
  10. 10-infectious-diseases
    6 主题
  11. 11-immunity
    10 主题
课 3, 主题 5
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3-2-factors-that-affect-enzyme-action AS rate-substrate-concentration

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Exam code:9700

Rate: substrate concentration

  • The greater the substrate concentration, the higher the rate of reaction:

    • As the number of substrate molecules increase, the likelihood of enzyme-substrate complex formation increases

    • If the enzyme concentration remains fixed but the amount of substrate is increased, past a certain point all available active sites eventually become saturated

      • At this point, any further increase in substrate concentration will not increase the reaction rate

    • When the active sites of the enzymes are all full, any substrate molecules that are added have nowhere to bind in order to form an enzyme-substrate complex

      • This means the reaction rate will not increase any further until active sites become free again

  • For this reason, in the graph below there is a linear increase in reaction rate as substrate is added, which then plateaus when all active sites become occupied

Graph showing reaction rate against substrate concentration. Curve rises, then plateaus indicating enzyme saturation. Text explains active site saturation.
The effect of substrate concentration on the rate of an enzyme-catalysed reaction

Examiner Tips and Tricks

If substrate concentration is continually increased but enzyme concentration is kept constant, there eventually comes a point where every enzyme active site is working continuously.

At this point, the substrate molecules are effectively ‘queuing up’ for an active site to become available. At this stage, the enzyme is working at its maximum possible rate, known as Vmax (V stands for velocity).

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