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Biology AS CIE

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  1. 1-cell-structure
    10 主题
  2. 2-biological-molecules
    19 主题
  3. 3-enzymes
    13 主题
  4. 4-cell-membranes-and-transport
    16 主题
  5. 5-the-mitotic-cell-cycle
    8 主题
  6. 6-nucleic-acids-and-protein-synthesis
    9 主题
  7. 7-transport-in-plants
    11 主题
  8. 8-transport-in-mammals
    16 主题
  9. 9-gas-exchange
    6 主题
  10. 10-infectious-diseases
    6 主题
  11. 11-immunity
    10 主题
课 3, 主题 6
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3-2-factors-that-affect-enzyme-action AS rate-enzyme-concentration

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Exam code:9700

Rate: enzyme concentration

  • Enzyme concentration affects the rate of reaction

  • The higher the enzyme concentration in a reaction mixture:

    • The greater the number of active sites available

    • The greater the likelihood of enzyme-substrate complex formation

  • As long as there is sufficient substrate available, the initial rate of reaction increases linearly with enzyme concentration

  • If the amount of substrate is limited, at a certain point any further increase in enzyme concentration will not increase the reaction rate as the amount of substrate becomes a limiting factor

Graph showing rate of reaction versus enzyme concentration, depicting linear increase due to more active sites and sufficient substrate availability.
The effect of enzyme concentration on the rate of an enzyme-catalysed reaction

Examiner Tips and Tricks

Always mention substrate availability when explaining the effect of increasing enzyme concentration.

A common mistake is to say that “more enzymes always increase the rate” — but this is only true while there is enough substrate present. In exams, you should state that if substrate becomes limiting, the rate will level off even if enzyme concentration keeps increasing.

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