Biology AS CIE
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1-cell-structure10 主题
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1-2-cells-as-the-basic-units-of-living-organisms AS viruses
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1-2-cells-as-the-basic-units-of-living-organisms AS prokaryotic-v-eukaryotic-cells
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1-2-cells-as-the-basic-units-of-living-organisms AS the-vital-role-of-atp
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1-2-cells-as-the-basic-units-of-living-organisms AS animal-and-plant-cells
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1-2-cells-as-the-basic-units-of-living-organisms AS eukaryotic-cell-structures-and-functions
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1-1-the-microscope-in-cell-studies AS calculating-actual-size
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1-1-the-microscope-in-cell-studies AS resolution-and-magnification
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1-1-the-microscope-in-cell-studies AS eyepiece-graticules-and-stage-micrometers
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1-1-the-microscope-in-cell-studies AS magnification-calculations
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1-1-the-microscope-in-cell-studies AS the-microscope-in-cell-studies
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1-2-cells-as-the-basic-units-of-living-organisms AS viruses
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2-biological-molecules19 主题
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2-4-water AS water-and-the-hydrogen-bond
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2-4-water AS the-role-of-water-in-living-organisms
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2-3-proteins AS collagen
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2-3-proteins AS haemoglobin
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2-3-proteins AS globular-and-fibrous-proteins
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2-3-proteins AS protein-shape
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2-3-proteins AS the-four-levels-of-protein-structure
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2-3-proteins AS amino-acids-and-the-peptide-bond
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2-2-carbohydrates-and-lipids AS phospholipids
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2-2-carbohydrates-and-lipids AS triglycerides
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2-2-carbohydrates-and-lipids AS cellulose
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2-2-carbohydrates-and-lipids AS starch-and-glycogen
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2-2-carbohydrates-and-lipids AS the-glycosidic-bond
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2-2-carbohydrates-and-lipids AS reducing-and-non-reducing-sugars
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2-2-carbohydrates-and-lipids AS covalent-bonds-in-polymers
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2-2-carbohydrates-and-lipids AS biological-molecules-key-terms
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2-1-testing-for-biological-molecules AS testing-for-non-reducing-sugars
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2-1-testing-for-biological-molecules AS the-benedicts-test
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2-1-testing-for-biological-molecules AS biological-molecule-tests
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2-4-water AS water-and-the-hydrogen-bond
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3-enzymes13 主题
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3-2-factors-that-affect-enzyme-action AS enzyme-activity-immobilised-v-free
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3-2-factors-that-affect-enzyme-action AS enzyme-inhibitors
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3-2-factors-that-affect-enzyme-action AS vmax-and-the-michaelis-menten-constant
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3-2-factors-that-affect-enzyme-action AS rate-inhibitor-concentration
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3-2-factors-that-affect-enzyme-action AS rate-substrate-concentration
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3-2-factors-that-affect-enzyme-action AS rate-enzyme-concentration
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3-2-factors-that-affect-enzyme-action AS rate-ph
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3-2-factors-that-affect-enzyme-action AS rate-temperature
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3-1-mode-of-action-of-enzymes AS colorimetry
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3-1-mode-of-action-of-enzymes AS measuring-enzyme-activity
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3-1-mode-of-action-of-enzymes AS how-enzymes-work
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3-1-mode-of-action-of-enzymes AS enzyme-action
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3-1-mode-of-action-of-enzymes AS enzymes
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3-2-factors-that-affect-enzyme-action AS enzyme-activity-immobilised-v-free
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4-cell-membranes-and-transport16 主题
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4-2-movement-into-and-out-of-cells AS comparing-osmosis-in-plants-and-animals
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4-2-movement-into-and-out-of-cells AS osmosis-in-animals
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4-2-movement-into-and-out-of-cells AS osmosis-in-plant-cells
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4-2-movement-into-and-out-of-cells AS estimating-water-potential-in-plants
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4-2-movement-into-and-out-of-cells AS investigating-surface-area
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4-2-movement-into-and-out-of-cells AS surface-area-to-volume-ratios
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4-2-movement-into-and-out-of-cells AS investigating-diffusion
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4-2-movement-into-and-out-of-cells AS investigating-transport-processes-in-plants
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4-2-movement-into-and-out-of-cells AS endocytosis-and-exocytosis
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4-2-movement-into-and-out-of-cells AS active-transport
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4-2-movement-into-and-out-of-cells AS osmosis
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4-2-movement-into-and-out-of-cells AS diffusion
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4-1-fluid-mosaic-membranes AS cell-signalling
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4-1-fluid-mosaic-membranes AS the-cell-surface-membrane
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4-1-fluid-mosaic-membranes AS components-of-cell-surface-membranes
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4-1-fluid-mosaic-membranes AS the-fluid-mosaic-model
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4-2-movement-into-and-out-of-cells AS comparing-osmosis-in-plants-and-animals
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5-the-mitotic-cell-cycle8 主题
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5-2-chromosome-behaviour-in-mitosis AS observing-mitosis
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5-2-chromosome-behaviour-in-mitosis AS the-stages-of-mitosis
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5-1-replication-and-division-of-nuclei-and-cells AS how-tumours-form
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5-1-replication-and-division-of-nuclei-and-cells AS the-role-of-stem-cells
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5-1-replication-and-division-of-nuclei-and-cells AS the-role-of-telomeres-
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5-1-replication-and-division-of-nuclei-and-cells AS the-cell-cycle
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5-1-replication-and-division-of-nuclei-and-cells AS mitosis
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5-1-replication-and-division-of-nuclei-and-cells AS chromosome-structure
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5-2-chromosome-behaviour-in-mitosis AS observing-mitosis
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6-nucleic-acids-and-protein-synthesis9 主题
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6-2-protein-synthesis AS gene-mutations
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6-2-protein-synthesis AS transcription
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6-2-protein-synthesis AS constructing-polypeptides
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6-2-protein-synthesis AS the-universal-genetic-code
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6-2-protein-synthesis AS from-gene-to-polypeptide
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6-1-structure-of-nucleic-acids-and-replication-of-dna AS the-structure-of-rna
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6-1-structure-of-nucleic-acids-and-replication-of-dna AS semi-conservative-dna-replication
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6-1-structure-of-nucleic-acids-and-replication-of-dna AS the-structure-of-dna
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6-1-structure-of-nucleic-acids-and-replication-of-dna AS nucleotides
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6-2-protein-synthesis AS gene-mutations
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7-transport-in-plants11 主题
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7-2-transport-mechanisms AS phloem-mass-flow
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7-2-transport-mechanisms AS the-sucrose-loading-mechanism
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7-2-transport-mechanisms AS movement-in-the-phloem
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7-2-transport-mechanisms AS xerophytic-plant-leaf-adaptations
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7-2-transport-mechanisms AS water-and-the-transpiration-pull
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7-2-transport-mechanisms AS transpiration-in-plants
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7-2-transport-mechanisms AS water-and-mineral-ion-transport-in-plants
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7-1-structure-of-transport-tissues AS phloem-sieve-tube-elements
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7-1-structure-of-transport-tissues AS xylem-vessels-elements
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7-1-structure-of-transport-tissues AS xylem-and-phloem-distribution
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7-1-structure-of-transport-tissues AS plant-transverse-sections
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7-2-transport-mechanisms AS phloem-mass-flow
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8-transport-in-mammals16 主题
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8-3-the-heart AS heart-action
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8-3-the-heart AS the-cardiac-cycle
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8-3-the-heart AS the-walls-of-the-heart
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8-3-the-heart AS structure-of-the-heart
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8-2-transport-of-oxygen-and-carbon-dioxide AS the-bohr-shift
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8-2-transport-of-oxygen-and-carbon-dioxide AS the-oxygen-dissociation-curve
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8-2-transport-of-oxygen-and-carbon-dioxide AS plasma-and-carbon-dioxide
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8-2-transport-of-oxygen-and-carbon-dioxide AS the-chloride-shift
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8-2-transport-of-oxygen-and-carbon-dioxide AS red-blood-cells-haemoglobin-and-oxygen
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8-1-the-circulatory-system AS blood-tissue-fluid-and-lymph
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8-1-the-circulatory-system AS the-role-of-water-in-circulation
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8-1-the-circulatory-system AS cells-of-the-blood
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8-1-the-circulatory-system AS blood-vessels-structures-and-functions
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8-1-the-circulatory-system AS observing-and-drawing-blood-vessels
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8-1-the-circulatory-system AS the-main-blood-vessels
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8-1-the-circulatory-system AS circulatory-systems
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8-3-the-heart AS heart-action
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9-gas-exchange6 主题
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9-1-the-gas-exchange-system AS gas-exchange-processes
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9-1-the-gas-exchange-system AS structures-and-functions-of-the-gas-exchange-system
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9-1-the-gas-exchange-system AS recognising-structures
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9-1-the-gas-exchange-system AS recognising-tissues
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9-1-the-gas-exchange-system AS distribution-of-tissues
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9-1-the-gas-exchange-system AS the-human-gas-exchange-system
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9-1-the-gas-exchange-system AS gas-exchange-processes
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10-infectious-diseases6 主题
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11-immunity10 主题
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11-2-antibodies-and-vaccination AS vaccination-to-control-disease
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11-2-antibodies-and-vaccination AS how-vaccines-work
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11-2-antibodies-and-vaccination AS types-of-immunity
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11-2-antibodies-and-vaccination AS uses-of-monoclonal-antibodies
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11-2-antibodies-and-vaccination AS making-monoclonal-antibodies
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11-2-antibodies-and-vaccination AS antibodies
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11-1-the-immune-system AS memory-cells-and-immunity
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11-1-the-immune-system AS primary-immune-response
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11-1-the-immune-system AS antigens
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11-1-the-immune-system AS phagocytes
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11-2-antibodies-and-vaccination AS vaccination-to-control-disease
1-1-the-microscope-in-cell-studies AS resolution-and-magnification
Exam code:9700
Resolution & magnification
Magnification
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Magnification is the number of times that a real-life specimen has been enlarged to give a larger view/image
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E.g. a magnification of x100 means that a specimen has been enlarged 100 times to give the image shown
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A light microscope has two types of lens which allow it to achieve different levels of magnification:
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An eyepiece lens, which often has a magnification of x10
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A series of objective lenses, each with a different magnification, e.g. x4, x10, x40 and x100
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To calculate the total magnification of a specimen being viewed, the magnification of the eyepiece lens and the objective lens are multiplied together:
Total magnification = eyepiece lens magnification x objective lens magnification
Resolution
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The resolution of a microscope is its ability to distinguish two separate points on an image as separate objects; this determines the ability of a microscope to show detail
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If resolution is too low then two separate objects will be observed as one point, and an image will appear blurry, or an object will not be visible at all
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The resolution of a microscope limits the magnification that it can usefully achieve; there is no point in increasing the magnification to a higher level if the resolution is poor
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The resolution of a light microscope is limited by the wavelength of light
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Visible light falls within a set range of light wavelengths; 400-700 nm
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The resolution of a light microscope cannot be smaller than half the wavelength of visible light
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The shortest wavelength of visible light is 400 nm, so the maximum resolution of a light microscope is 200 nm
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E.g. the structure of a phospholipid bilayer cannot be observed under a light microscope due to low resolution:
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The width of the phospholipid bilayer is about 10 nm
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The maximum resolution of a light microscope is 200 nm, so any points that are separated by a distance of less than 200 nm, such as the 10 nm phospholipid bilayer, cannot be resolved by a light microscope and therefore will not be distinguishable as separate objects
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Electron microscopes have a much higher resolution, and therefore magnification, than light microscopes as electrons have a much smaller wavelength than visible light
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Electron microscopes can achieve a resolution of 0.5 nm
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Examiner Tips and Tricks
Don’t assume that increasing magnification will let you see more detail — it won’t if the resolution is too low.
Remember: magnification makes things bigger, resolution makes things clearer.
Responses