2-4-4-diffusion

Diffusion

• Diffusion is a type of transportation that occurs across the cell membrane

• It can be defined as:

The net movement of molecules or ions from a region of higher concentration to a region of lower concentration

• The molecules or ions move down a concentration gradient

• Movement is random and is caused by the natural kinetic energy of the molecules or ions

Diffusion across the cell membrane

• As a result of diffusion, molecules or ions tend to reach an equilibrium situation (given sufficient time), where they are evenly spread within a given volume of space

Factors influencing the rate of diffusion

• The rate at which a substance diffuses across a membrane depends on several factors

  Factor	How the factor affects the rate of diffusion
  Steepness of the concentration gradient	A greater difference in concentration means more molecules move from high to low concentration This increases the net rate of diffusion across the membrane
  Temperature	Higher temperatures give molecules more kinetic energy, so they move faster This results in a higher rate of diffusion
  Surface area	A larger surface area allows more molecules to diffuse at once Folding (e.g. microvilli, cristae) increases surface area In larger cells, a lower surface area to volume ratio slows diffusion
  Properties of molecules or ions	Large molecules diffuse more slowly as they need more energy Uncharged, non-polar molecules diffuse directly through the bilayer Non-polar molecules diffuse faster than polar ones

Facilitated diffusion

• Certain substances cannot diffuse through the phospholipid bilayer of cell membranes. These include:

  • Large polar molecules such as glucose and amino acids

  • Ions such as sodium ions (Na⁺) and chloride ions (Cl^–)

• These substances can only cross the phospholipid bilayer with the help of certain proteins

• This form of diffusion is known as facilitated diffusion

• There are two types of proteins that enable facilitated diffusion:

  • Channel proteins

  • Carrier proteins

• They are highly specific (they only allow one type of molecule or ion to pass through)

Channel proteins

• Channel proteins are water-filled pores

• They allow charged substances (eg. ions) to diffuse through the cell membrane

• The diffusion of these ions does not occur freely, most channel proteins are ‘gated’, meaning that part of the channel protein on the inside surface of the membrane can move in order to close or open the pore

• This allows the channel protein to control the exchange of ions

Carrier proteins

• Unlike channel proteins which have a fixed shape, carrier proteins can switch between two shapes

• This causes the binding site of the carrier protein to be open to one side of the membrane first, and then open to the other side of the membrane when the carrier protein switches shape

• Net diffusion of molecules or ions into or out of a cell will occur down a concentration gradient (from an area containing many of that specific molecule to an area containing less of that molecule)